Test 2 Survivability of Fungi
To find out how the samples are able to resist the
survivability of fungi, a sample swatch survival test was carried out using two
test organisms (Aspergillus niger and Trichoderma viridae. Starting from 48 hours
to 260 hours of incubation at 270C, the survival of the known fungi
in all the 4 samples was assayed over a constant period of time, every 24
hours. Results indicate that the survivability of fungi was more or less the
same on all the four test samples.
From this test, it can be concluded that the antifungal
resistance of bamboo, cotton and 50/50% bam/cot are equally efficient, when
compared with rayon. Generally, all the four samples supported the growth of T.
Viridae and resisted the growth of A. niger.
Table - 5 Survival of fungi - Aspergillus
niger (Before wear of socks)
|
Samples
(Socks)
|
Initial
0.1 ml inocula
|
No. of Colonies (102 cfu/ml)
|
|
Day-1
|
Day-2
|
Day-3
|
Day-4
|
Day-5
|
|
100% Bamboo
|
7*
|
5
|
2
|
-
|
-
|
-
|
|
50/50% Bam/Co
|
4
|
-
|
-
|
-
|
-
|
|
100% Cotton
|
5
|
1
|
-
|
-
|
-
|
|
100% Viscose
|
7
|
5
|
8
|
13
|
14
|
* 102 cfu/ml
Table -6 Survival of fungi - Trichoderma
viridae (Before wear of socks)
|
Samples
(Socks)
|
Initial
0.1 ml inocula
|
No. of Colonies (102 cfu/ml)
|
|
Day-1
|
Day-2
|
Day-3
|
Day-4
|
Day-5
|
|
100% Bamboo
|
19*
|
34
|
35
|
28
|
23
|
20
|
|
50/50% Bam/co
|
29
|
28
|
20
|
19
|
19
|
|
100% Cotton
|
38
|
38
|
35
|
35
|
28
|
|
100% Viscose
|
39
|
30
|
34
|
36
|
20
|
* 102 cfu/ml
Test 3 - Determination Of Rate Of Growth And Comparison
Between Four Samples
The growth rate of bacteria (E. coli and S. aureus)
and fungi (T. Viridae and A. niger) were compared when grown on
the four test samples as substrates. The results are presented in Table.7. This
test proves that the growth rate of organisms on bamboo as a substrate is less,
when compared with cotton and rayon. The natural antimicrobial effect of bamboo
does not allow the multiplication of bacteria and fungi and ultimately proves
to be both bacteria static and fungi static. The growth rate of microorganisms
on 100% cotton is same as rayon. On 50/50% bam/cot, the growth rate of organism
is less when compared with cotton.
Table -7 Comparison of the growth
rate of bacteria and fungi
|
S.No.
|
Sample
|
Bacterial growth rate
|
Fungal growth rate
|
|
E. Coli
bac - 1
|
S. Aureus
bac - 2
|
A. Niger
Fungi - 1
|
T. Viridae
fungi - 2
|
|
1
|
100% Bamboo
|
+
|
++
|
+
|
++
|
|
2
|
100% Cotton
|
++
|
+++
|
++
|
+++
|
|
3
|
50/50% Bam/Co
|
+
|
++
|
+
|
+++
|
|
4
|
100% Viscose
|
+++
|
+++
|
++
|
+++
|
Note
+ - Moderate Growth Rate
++ - High Growth Rate
+++ - Very High Growth Rate