|
We initiated cranberry cell suspension culture by transferring callus to liquid media of the same composition as the callus medium and gently agitating the suspension on a horizontal shaker at 150 rpm and 20oC (Figures 2 and 3). We have found out that the growth of biomass of cranberry cell suspension culture in WP and MS liquid media were greater than B5 liquid medium (Figure 4).
Interestingly, anthocyanin content of cranberry cell suspension culture in MS liquid medium was
higher than in WP liquid medium (Figure 5). However, the flavonol content of cranberry cell
suspension culture in WP liquid medium was higher than in MS liquid medium. 
Figure 2. Initiation of Suspension Cell Culture
Suspension cell cultures were initiated by transferring fresh mass callus to 15 ml of WP liquid medium and incubated on a rotary shaker at 150 rpm, 20 oC 
Figure 3. Examination of Different Media (WP, B5, and MS)
Suspension cell cultures were under a continuous photosynthetic photon flux of 25M m-2s-1 provided by cool white fluorescent lamps (F40CW-RS, General Electric Company, USA) plus continuous red light at a photon fluence rate of 12 M m-2s-1, was obtained from six 40-w bulbs (F48T12/R-660/HO, Red, General Electric Company, USA) filtered through a red plastic sheet (Roscolux color filter # 27, ROSCO Laboratories, Port Chester, NY)
|