Figure 4. Growth of Cranberry Suspension Cells in Different Media (WP, B5, and MS)
Growth was evaluated quantitatively in term of biomass, Fr.Wt. (g/flask) under different media. Cells were separated from cell suspension medium by vacuum filtration through 3 mm Whatman filter paper, just until the point when free liquid was no longer expressed, and Fr.Wt. was immediately recorded.
Figure 5. Anthocyanin and Flavonol Contents in Cranberry Suspension Cell Culture
Comparison of Different Liquid Media
Experimental Approach
We used the following approach to assess the potential value of using cranberry cell suspensions as a production source of environmentally friendly red dyes:
Preparation of cranberry extract from the red cranberry fruits containing the anthocyanin and
flavonol pigments;
Extract prepared by crushing the cranberry in frozen form by the use of liquid nitrogen and formation of the solution by common solvents (HCl, CH3OH and water);
Suitable and industrially accepted pre-treatments of the fabrics to be used for dyeing from the cranberry extract;
Dyeing of the most widely used polyamide fabrics (Nylon-66 and wool) by the exhaustion method of acid dyeing;
Determination of the exhaustion curves of the dyeing process;
Comparison of the dyeing exhaustion curves with the standard acid dyes having the wavelength of maximum absorption in the range of the wavelength of maximum absorption of the cranberry extract;
Establishment of the equations between the exhaustion of dye and the concentration of the dye
solution;
Matching of the shade of the dyed fabrics by the use of spectrophotometry and computer color
matching.